Abstract:
In this study the stability of Atenolol in solid and liquid forms was
investigated, towards high temperature, sunlight, UV light, some
common excipients and hydrolysis in acidic media and alkaline
media, using high performance liquid chromatography ( HPLC ).the
results obtained were compared with the UV spectrophotometric
method. A new stability indicating ( HPLC) method was validated
for assaying Atenolol.
It was found that Atenolol was thermally stable in solid and
aqueous solution form up to70oC. On exposure to UV radiation,
however,
the
solution
form
was
unstable,
and
the photo
scanning of Atenolol solution with different pH values
decomposition showed first order kinetic reaction.
UV
showed bathochromic (red) shift as ƛ
max
was changed to longer
wavelength with increased pH of the solution.
Atenolol hydrolysis in acidic and alkaline media showed also first
order reaction kinetic and the reaction rate was temperature
dependant. Both hydrolyzed compounds from acidic and alkaline
media have same retention time in the chromatograms.
Comparing the results of hydrolysis obtained from ( HPLC ) with
that obtained by UV. Spectrophotometric analysis, the UV results
showed no hydrolysis .The method is not stability indicating
method.
A new analytical high performance liquid chromatography ( HPLC )
method was validated for Quantification assay for Atenolol with
IVaccuracy 99.55% and linearity 99.99% and compared with (USP)
method.
