Measurement of Some Coagulation Factors in Normal Pregnant Females in Khartoum State

Abstract

Normal pregnancy is characterized by impressive changes in the activating and inhibitory pathways of coagulation and fibrinolysis resulting in an accelerated, but well balanced, process of thrombin formation and resolution. The fibrinolytic activity of the blood progressively decreases during pregnancy, and in the last month it is often barely demonstrable not even after venous stasis. The aim of this study is to investigate changes and to establish reference values in coagulation fibrinolysis and hemodynamic during normal pregnancy, and to determine different parameters of blood coagulation in the threetrimester of gastiation . (The question remains whether the coagulation parameters increase during pregnancy.) . This is anylatical case control study which was conducted in khartoum state during the period from May to August 2013 to evaluate activated partial thromboplastin time " prothrombin time and fibrinogen level during normal pregnancy . Hundred blood sample were collected from pregnant females according to inclusion criteria and consider as case , and fifty sample were collected frome non pregnant females and considered as control 5 ml of sample collected in vacutaner tube which contain (3.2) trisodium citrate solution for anticoagulation. Then the contents were mixed and centrifuged at 3000 round/ min for 15 minutes for prepration of platelets poor plasma. Which tested for coagulation testes by using coagulometer instrument. The results were analyzed by independent T test the SPSS computer programe. The results of cases revealed that the mean of APTT was=31.8±3.8 / sec , PT= 15.7±1.4/ sec and fibrinogen level = 467± 227/mg/dl. The results of control group revealed that the mean of PTT=29.8± 4.11/ sec , PT=13.3± .94/ sec and fibrinogen level=292± 70.6/mg/dl. The study found that the mean of PT in age group between 15 – 25 years old was 15.5± 1.6sec and the P > 0.478. The mean of PTT was 31.9± 3.3sec and the p > 0.973. The mean of Fibrinogen was 458.6±224.3mg/dl and the P > 0.955. The result found that the mean of PT in the first trimester was 15.3±.9sec in second trimester was 15.9±1.3sec and in third trimester was 15.7±1.6sec with P. value > 0.597. The mean of PTT in first trimester was 27.4±3.7sec, in second trimester was 31.3±3.9sec and in third trimester 32.5±3.3sec with P.value > 0.009. The mean of Fibrinogen in first trimester was 502±229.9mg/dl, in second trimester was 468±213.4mg/dl, and in third trimester 471±244.0mg/dl the P > 0.953. Over all the results were showed significant increase in activated partial thromboplastin time and fibrinogen level when compared with control group with p value >0;00 and there was significant chang noticed in the different trimester of pregnant period.