Association of Legionella pneumophila with Chest Infection among patients Attending Selected Hospitals in Khartoum State

Abstract

The present study aimed to determine the rate of detection of legionella pneumophila(L. pneumophila) among patients who presented to selected hospitals in Khartoum State with pneumonia/ chest infection. During the period from September 2012 to September 2013 of a total of 300 patients were selected randomly among patients attending Omdurman teaching Hospital, Jafar Ibnaof Hospital, Elswedy Hospital and some health centers (Elhikmma Center, Wadnobawi Center, and special clinics) with chest infection/ pneumonia. The mean age of the patients was 36 years range. Male were more than female (55%, 45%) respectively. Venous blood was collected from each individual under study allowed to clot and after cloting retraction, centrifuged and the serum was separated and stored until used. The serum samples were later tested for L.pneumophila IgG antibodies by ELISA (Euroimmun). DNA extraction was done on serum by (G-DEXIIb-kits) Intron Biotechnology. PCR was then performed for amplification of mip gene by Real Time PCR by using Real MODTM Green Real Time PCR master mix kit – Intron Biotechnology. The overall seroprevalence of L.pneumophila IgG was 22.7% of the total number collected specimens. 100 were tested for L.pneumophila DNA by PCR and 10 (10%) were found positive.