The Antimicrobial Effect of Large Baladi and Exotic Poultry Breeds on Egg Yolk IgY

Abstract

This study was conducted at the college of Veterinary Medicine Sudan University of Science and Technology ,Kuku to explore The antimicrobial effect of large baladi and exotic poultry breeds egg yolk on E.coli and Pseudomonas bacteria among three dilution (1:0.1,1:1and 1:10). A total of 120 eggs were randomly collected from each breed from different farms. The yolk was separated from the albumen after cracking the shell and kept at sterile urine containers. The yolk was diluted to (1:0.1,1:1 and 1:10) respectively then heated and centrifuged the supernatant was used. The petri dishes used in inoculation were holed with 6 wells after agar solidification. Each 3 wells deal with different breed and each one from the three wells deals with target dilution. About 240 replicate were carried out for each concentrate, 180 replicates for each breed and 180 replicate for each organism. The study revealed that there is a significant differences in the inhibition zones between local breed which record an average of (14.03 ± 0.18) (9.21 ± 0.14) (4.71 ± 0.11) and exotic breed (11.57 ± 0.18) (7.65 ± 0.14)( 3.81 ± 0.11) at (P<0.05) in dilution (1:0.1,1:1and 1:10) respectively. also there is significantly differences between E.coli(-ve) which record (9.18 ± 0.18)( 6.15 ± 0.14) (2.85 ± 0.11) and pseudomonas (+ve) (16.43 ± 0.18) (10.71 ± 0.14)( 5.66 ± 0.11) at (P<0.05) in dilution (1:0.1,1:1and 1:10) respectively.