Bacteriological status of sheep carcasses in slaughter house in Khartoum state

Abstract

The purpose of this work was to study the degree of superficial bacterial contamination of ovine carcasses at El-kadaru, Elshaheed and Elsabaloga slaughterhouse (Khartoum - Sudan) quantitatively by counting the total viable and, qualitatively by the isolation and identification of Listeria monocytogenese, E. coli and Salmonella. Carcasses were examined just after skinning, eviscerating and washing for the total viable count.After skinning for isolation of Listeria spp, E. coli and salmonella. Swab sampling was used. Viable count was done according to the known conventional methods. The isolation of Listeria ,E.coli and Salmonella was don on enriched and . selective media At Elkadaro slaughterhouse: The highest contamination level was recorded in the evisceration stage at foreleg (3.19±1.34Log10 CFU/cm2), whereas the lowest contamination was recorded in the skinning stage at rump site. (2.3957±0.54Log10 CFU/cm2).At the skinning point the highest contamination levels recorded were in the site of the flank (2.85±1.18Log10 CFU/c m2),whereas the lowest contamination levels recorded were in the site of the rumb (2.57±0.354Log10

CFU/c m2) .At the evisceration point the highest contamination levels recorded were in the site of the fore leg (3.19±1.34Log10 CFU/c m2 ) whereas the lowest contamination levels recorded were in the site of the rump (2.77±1.22 Log10 CFU/c m2 ). At the washing stage the highest contamination levels recorded were in the site of the flank (3.07±1.220 Log10 CFU/c m2(,whereas the lowest contamination recorded were in the site of the froe leg (3.04±1.15 Log10 CFU/c m2 (.On hands of workers during the process of slaughtering the highest contamination levels recorded at washing point (3.39±0.71 Log10 CFU/c m2 ) , whereas the lowest contamination recorded at skinning point (3.23±0. 59 Log10 CFU/c m2 ).The bacteriological examination on knives used during skinning and evisceration stages respectively revealed that the highest contamination levels recorded at evisceration stage (4.77±2.73Log10 CFU/c m2 ) , whereas the lowest contamination recorded in the skinning . ( point (4.33±2.78Log10 CFU/c m2 At Elshaheed slaughterhouse: At the skinning point the highest contamination levels recorded in the site of the flank (4.50±2.07 Log10 CFU/c m2(, whereas the lowest contamination recorded in the site of the foreleg (2.25±1.53Log10 CFU/c m2).But the evisceration point at Elshaheed slaughterhouse the highest contamination levels recorded in the site of the rump (4.66±1.75Log10 CFU/c m2), whereas the lowest contamination .(recorded in the site of the flank (4.01±2.66Log10 CFU/c m2

The results of the total viable count indicate that the .contamination was increased during slaughter line Six Listeria sppwere isolated from examined 600samples (1%),42E. coli bacteria were isolated from 300samples(14%) Salmonella was not isolated from examined 200 examined samples. The present results reflect poor conditions of slaughtering and handling of carcasses, as well as hygiene deficiencies as high prevalence of E. coli (14%) and Listeria .spp(1%) represents a real danger for the consumer