Exploration of Singlet Oxygen Emitted After Irradiation of Dibenzocyanine 45, MethyleneBlue and Rhodamine 6G Dyes Using Lasers with Different Wavelengths

Abstract

The aim of this research was to explore the singlet oxygen emitted after irradiation Dibenzocyanine 45, Methylene Blue and Rhodamine 6G dyes after irradiation using two lasers with different wavelengths, so that the singlet oxygen can be used in photodynamic therapy (PDT). Three samples of DDTTC 45 were prepared by dissolved in propanol, methanol and acetone respectively. Two samples of Methylene blue prepared by dissolved in methanol and ethanol. One sample of Rhodamin 6G was dissolved in methanol. UV visible spectrometer was used to record the absorption spectra for the solvents and dyes. Two laser sources were used to irradiate the samples. Firstly diode laser (532nm) with output powers of 100 mW, secondly the diode laser (671nm) with output power of 100 mW. USP2000 spectrometer connected to PC, was used to collect and record the emission spectra after different exposure times. The results showed that the most efficient sample to produce the singlet oxygen was the DDTTC 45 dissolved in acetone after irradiation by the diode laser 671 nm with exposure time of six minutes which indicated a peak of singlet oxygen at (703 nm). It is worth to mention that, no evidence was noticed for a singlet oxygen production from the other samples.