Demonstration of Thyroid Tumors Using Immunohistchemical Methods

Abstract

This is a retrospective study conducted in Khartoum State, during the period from March to August 2009 to demonstrate the expression of cytokeratin and vimentin intermediate filament among different grades of thyroid tumor, and to find out the association between amyloidosis and thyroid cancer, on other hand to compare between congo red and metachromatic stains in demonstration of amyloid. Sixty specimens of paraffin wax were collected laboratories administration with different grade of thyroid tumor. The result showed CytokeratinMNF116 was expressed in 31 (51.7%) of different grade of thyroid tumor. Among these 10 (16.66%) samples of papillary carcinoma, 10 (16.66%) samples of multinodular goitre, and 11 (18.33%) samples of follicular adenoma. The expression of Vimentin 9 found in 48 (80%), eight (13.33%) samples were follicular carcinomas, 10 (16.66%) samples papillary carcinoma, 10 (16.66%) samples were multinodular goitre, 11(18.33%) samples follicular adenoma, nine (15%) samples were hashimoto’s. Amyloidogenic materials were found to be associated with the medullary carcinoma. Thus investigation of medullary carcinoma for amyloid deposition is highly recommended. Further advanced studies are needed to disclose the relation between the amyloid and thyroid cancer according to the causes. From the previous results the expression of cytokeratinMNF116 was showed in the papillary and follicular carcinoma, while the expression of vimentin9 was detected only in the papillary carcinoma, also hasimoto’s disease show expression for vimentin9. So further studies on tumor marker that associated with thyroid tumor should b to facilitate the differentiation between different thyroid tumors.