Detection of Extended Spectrum Beta-lactamase in Klebsiella pneumoniae Isolated from Sputum

Abstract

The aim of this study was to detect extended spectrum ß-lactamase (ESBL) in Klebsiella pneumoniae isolated from sputum. A total of 100 sputum specimens were collected from Alshaab Teaching Hospital during April to June 2012. Bacteriological tests of sputum specimens were performed for Klebsiella pneumoniae including inoculation on MacConkey’s agar and blood agar. The identity of the isolates was confirmed by biochemical tests. Out of 100 sputum specimens; 21 (21%) Klebsiella pneumoniae was recovered from patients with different age groups. Susceptibility test and extended spectrum ß-lactamase production were carried out for each isolate. The isolates were resistance to nitrofurantoin, naldixic acid and co-trimoxazole and sensitive to imipenem. Screening tests for ESBL were performed against four types of third generation cephalosporin (3GC) and amoxyclave. Out of 21 isolates, 19 were sensitive and two were resistance. Double disc synergy test was done for the two isolates that were found resistance to the 3GC to detect ESBL an the result was positive .This study showed that Klebsiella pneumoniae considered to be one of ESBL producer.