Effect of Entamoeba histolytica on some Haematological Parameters

Abstract

This cross sectional hospital based study was carried out in Khartoum and Omdurman teaching hospitals to determine the effect of Entameba histolytica on hemoglobin level and total white blood cell with differential counts. In period between 10\5\2007 to 29\7\2007. The total sample size equal 150,were randomly selected from Sudanese patients of (77 males and 73 females), at different age groups, with and without clinical symptoms of intestinal amoebiasis ,they were divided in to three groups according to the questionnaire and microscopical stool examination (wet preparation and concentration techniques),these are non infected, infected before, and infected groups, other parasitic infections were excluded. Subsequently 150 blood samples were collected and analyzed using colorimetric method (0.02 ml capillary blood added to 4ml of drabkins solution)for haemoglobin measurement, white blood cell count using(0.02 ml of blood added to 0.38 ml of 2%Glacial acetic acid solution using Neubauer chamber for cell count).leishman stain for differential leukocytes count was added to a fixed thin blood film for 3-4 min then double volume of leishman buffer was added for 6-8 min. Haemoglobin level was found to be 12.12 g\dl, 12.08 g\dl and 11.25 g\dl in non infected, infected before and infected groups with E.histolytica respectively. The haemoglobin level decreased in infected group with E.histolytica and show highly significant differences between non-infected and infected group at p<0.01 level. Mean hemoglobin concentrations were found to be higher in males in all groups than in females, (12.6±.5g/dl[std=1.3]versus 11.2±.5 g/dl[std=1.2],p=.002).statistically significant at p<0.01 level. Mean leukocytes count in E.histolytica infected group (6300 c\cumm3), infected before (5300 c\cumm3) and non infected(4800 c\cumm3). When these results were compared, E.histolytica infected ‫,‪group had higher leukocytes count which exceed the normal mean value‬‬ ‫‪.there is very highly significant differences at p<0.01 level‬‬ ‫‪Differential leukocytes counts were increased in E.histolytica‬‬ ‫%09.1/%88.06(‪,(infected group in neurophils and eosinophils count‬‬ ‫‪Comparing to non-infected group(57.36%/0.92%),statistically significant‬‬ ‫‪at p<0.01 level. while lymphocytes, basophiles and monocytes count in‬‬ ‫‪E.histolytica infected group: (30.98%/6.06%/0.16%), were found to be‬‬ ‫‪.within the normal range‬‬ ‫