Isolation of Some Pathogenic Bacteria from Cow Raw Milk in Relation to Public Health at Khartoum State, Sudan.

Abstract

This study was conducted to isolate some pathogenic bacteria from raw milk and to assess the risk factors associated with milk consumption in the seven localities of Khartoum state (Khartoum, Jabal Awlia, Bahry, Sharg alnil, Omdurman, Ombadda, Karary) . Atotal of 102 samples of raw cow milk were collected in winter , summer and autumn seasons from December 2018 to October 2019 from milk equipment , udders of milking cows, and venders, the samples were analyzed and examined for detection of coliform bacteria and some pathogens contaminate raw milk causing milk borne diseases and effect on public health .The isolates were identified morphologically and biochemically and the results revealed that examined samples showed growth of Gram negative bacteria (233 isolates) and recorded , Escherichia spp. 75(32.19%), Klebsiella spp. 30(12.88%) Salmonella spp.15(6.44%), Shigellaspp. 20(8.58%), Proteus merabilis 37(15.88%), Proteus spp 29(12.45%).and Pseudomonas spp.7(11.58%) . According to the source of collection the isolates were 65(27.9%) from the udders , 93(40%) from equipment , and 75(32.1%) from vendors and this indicated high contamination in milk equipment . According to the seasons the isolates were 35(15.0%) in winter, 66(28.3%) in summer , and 132(56.7%) in autumn respectively .In conclusion the contamination of milk by bacteria is high because of this dairy workers need more awareness about sanitary measures. Keywords: pathogenic bacteria, cattle , raw milk.