Evaluation of Different Serological tests for diagnosis of Brucellosis in Camels and Male Goats Intended export in Sudan

Abstract

A total of 5700 samples of blood were collected from animals intended for export with a healthy appearance, with two thousand samples (2000) from male camels, seven hundred samples (700) from female camels, and three thousand samples (3000) of male goats. All animals are not vaccinated. In this research, four tests were performed on blood serum, namely examination of samples for the presence of antibodies to Brucella with a rapid test, which is the RosBengal RBPT test. The positive samples were examined by two confirmatory tests, namely the SAT and the BAPA test, and finally by the cELISA-bound enzyme competitive immunoassay. The results were as follows: The RBPT examination excluded 21% of male camels, 26% of female camels, and 14% of male goats. It is a very large percentage compared to other tests. The SAT test was less remote for positive animals, where the percentage of deported males was 12% and females 14.5%, and goats were 3.5%. As for the BAPA examination, it excluded 13% of male camels, 14.5% of females, and 3.5% of goats. As for the cELISA test, it confirmed the positive rate of 7.4% of male camels, 7.4% of female camels, and 1.5% of goats. Statistical analyses were carried out using the Statistical Package for Social Sciences version SPSS prevalence and cross-tabbing were computed. Associations in the Chi-square test and logistic regression model were deemed significant when p≤0.05. All tests consider to be significant because the P value ranged from .000 to .003 and all the results less than .05.