solation, Identification and Antibiotics Resistance Pattern of Staphylococcus aureus Isolated from Raw Sheep Milk Samples in Ghebaish City West Kordofan State- Sudan

Abstract

The diseases associated with Staphylococcal outbreaks are wide spread throughout the Sudan affecting both animals and humans. The most endemic types which affect the population health are those related to Staphylococcus aureus. Staphylococcus aureus is an important pathogen that can cause Staphylococcal Food Poisoning (SFP). Milk and dairy products are frequently contaminated with exterotoxigenic Staphylococcus aureus, which is often involved in Staphylococcal food poisoning. The objective of the present study was to evaluate the occurrence and antibiotic resistance of this bacterium. For this purpose sixty raw sheep milk samples were collected from different flocks throughout Ghebaish city. The samples were investigated for the presence of Staphylococci. The Staphylococcus aureus was isolated in Blood agar and Mannitol salt agar where typical and atypical colonies were selected and submitted to coagulase and other confirmatory tests.Out of 60 samples, (22) 36% isolates of Staphylococcus aureus were obtained and identified by biochemical tests.The results of sensitivity test reflected high antibiotic resistance rates of bacteria, Vancomycin (54%), Tetracycline (0%), Erythromycin (18%), Clindamycin (27%), Chloramphenicol (9%), Ceftrioxone (63%) and Methicillin (27%).It was concluded that S. aureus survives around animal environment causing infectious illnesses and begin to acquire resistance against different antibacterial agents and the presence of this pathogenic microorganism indicates a potential health hazard to those who consume milk from this region