Evalution Of Plasma Interleukin-8 (IL-8) Levels among Neonatal Sepsis Patients in some Selected Hospitals – Khartoum State - Sudan

Abstract

One of the most important factors playing important role in neonatal sepsis pathogenesis is cytokine release and the most effective cytokines that with proinflammatory characteristic is interleukin-8 (IL-8). This is a case-control study was aimed to evaluate the IL-8 level in Sudanese Septic neonates cases and in control subjects in Khartoum state during the period from November (2020) to May (2021). Fifty tow subjects were selected convincely in this study, with age varies from 1-13 days, mean of age was 3.4± 2.6. 26 subjects were septic neonates (13 males and 13 females) as case group and 26 subjects (13 males and 13 females) as healthy control group. Venous blood sample (1ml) was collected in EDTA container from each subject. IL-8 concentration was measured using Enzyme Linked ImmunosorbentAssay(ELISA).The data was analyzed using Statistical Package Social Science Programme (Version 20). The Means of IL-8 were 221±26.9, 111.1±28.7 in the case group and control group respectively. IL-8 level was significantly elevated in septic neonates than control group (P. value 0.005).In septic group there was no statistical correlation in IL-8 levels between early onset sepsis and late onset sepsis and between premature neonates and term neonates (P. value 0.66 and 0.77 respectively). Also there was no statistical correlation between level of IL-8 with gender and age (P. value was 0.67 and 0.15 respectively). The study concluded that IL-8 concentration may be useful as a diagnostic marker for neonatal sepsis.