Immunohistochemical Detection of Neuroblastoma Rat Sarcoma in Thyroid Tumors among Sudanese Patients

Abstract

This is descriptive retrospective case study conducted in Omdurman teaching hospital during the period from January 2017 to August 2017. The study aimed to detect the expression of N-RAS in thyroid tumors among Sudanese patients. Total of 40 fixed paraffin blocks previously diagnosed as thyroid tumors, 28 (70%) were malignant samples include, 16(40%) papillary thyroid cancer, 8(20%) follicular carcinoma and 4(10%) medullary thyroid carcinoma, and 12(30%) samples were benign include, 9(22.5)% samples multinodular goiter and 3(7.5%) samples werefollicular adenoma were enrolled in this study. One section of 3 microns was cut from each block and stained by immunohistochemicalmethod (avidinbiotin technique) for N-RAS detection. The data obtained were analyzed using SPSS computer program version 20. The patients ages ranged between 11-78 years with mean age of 39years, most of them 32(80%) were less than 50 years and the remaining 8(20%) were above 50 years. The sex of patient showed the majorly of them were female 28(70)%and 12(30%) were male. N-RAS showed positive expression in 10(25%)samples. All of them were malignant, 6(15%) samples were papillary thyroid cancer, 3(7.5%) samples were follicular carcinoma and 1(2.5%) sample weremedullary thyroid carcinoma, While 30(75%) samples were negative for N-RAS divided as 18(45%) in malignant and 12(30%) in benign, with significant correlation between N-RAS expression and thyroid tumorsdiagnosis (P.value0.036). The study concluded that N-RAS expression is associated with malignant form of thyroid tumors.