Multiplex Polymerase Chain Reaction Detection of Klebsiella pneumoniae Virulence Strains Isolated from Selected Drinking Water Sources in Khartoum State

Abstract

Good health depend on a clean, drinkable water supply. This mean that water must be free of pathogens, K.pneumoniae is found in the environment and as a harmless commensal, but is also a frequent nosocomial pathogen and the agent of specific human infections. This study is cross sectional study aimed to determine the frequency of virulence genes in the K.pneumoniae isolated from drinking water in Khartoum State (Omdurman, Khartoum, and Bahri) conducted in May to December 2019. A total of 100 isolates were obtained from Central Public Health Laboratory in Khartoum. These isolates were isolated from drinking water in Khartoum State and subcultures in Eosin Methylene Blue (EMB) media, the isolates were then identified by routine biochemical tests, 25 (25%) 0f isolates were identified as K.pneumoniae . Multiplex Polymerase Chain Reaction (MPCR) was performed to detect the virulence genes ( 213, ent , Kfu , MRK, mag , K2and RMP ). Most of the isolated bacteria were from tanke 12(48%), cooler 8(32%), and tap water 5 (20%). In addition to, the majority of them were from houses 10 (40%), dormitory 5 (20%), Cafeteria 6 (24%), pharmacy 2 (8%) and company 2 (8%). Most of them from Omdurman province 11 (44%), Khartoum 7 (28%) and Bahri 7 (28%). The multiplex PCR assay confirmed the presence of all virulence genes of K.pneumoniae ( 213, ent, Kfu ,MRK , mag , k2 and RMP ). The study concluded that a high prevalence of virulence strains of K.pneumonae in drinking water in Khartoum State. The water can also be a source of transmission of disease and drug resistant isolates. detection of virulence genes by Multiplex PCR was quite satisfactory.