Molecular detection of mecA gene in methicillin resistant Staphylococcus aureus isolated from food samples in Khartoum State

Abstract

This study was conducted in Khartoum State from August to November 2018. This study aimed to detect Staphylococcus aureus resistance genes isolated from food samples obtained from different retailers in Khartoum State, by conventional PCR assay. A total of four hundred samples were collected in this study. 84 (21%) of 400 of isolates were identified as S. aureus, 110 (27.5%) of 400 were identified as other than S. aureus and 206 (51.5%) of 400 yielded no growth. Oxacillin sensitivity test showed 31(36.9%) of 84 were Oxacillin resistant and 53 (63.1%) of 84 were Oxacillin sensitive. Finally PCR was done for the detection of resistace gene mecA in Staphylococcus aureus. The result of PCR assay for mecA gene was 15 of 84 (17%) positive and 69 (83%) negative. The distribution of mecA in types of samples was 17% in milk, 15% in fish, 20% in meat and 20% in cheese. furt