Phenotypic Detection of Extended Spectrum Beta- Lactamases in Enterobacteriaceae Isolated from Urine Specimens

Abstract

The aim of this study was to detect extended spectrum ß-lactamases (ESBLs) in Enterobacteriaceae isolated from urine. A total of 200 urine specimens were collected from International Medical Center Hospital from April to June 2018. Bacteriological tests of urine specimens were performed for Enterobacteriaceae, including inoculation on MacConkey agar and blood agar. The identity of the isolates was done by Vitek2 system. Out of 200 urine specimens; 27(13.5%) Enterobacteriaceae were recovered from patients with different ages. The test for extended spectrum ß-lactamases groups production was carried out for each isolate. Screening tests for ESBL were performed against cephalosporin group and Amoxyclave.11(5.5%) were Enterobacteriaceae ESBLs strains; 9 (4.5%) Escherichia coli ESBLs strain and 2 (1%) Klebsiella pneumonia ESBLs strain. Double disc synergy test was done for the eleven isolates that were found resistance to the cephalosporin group.