Synthesis and Characterization of Some Antibiotic-Silver Nanoparticles Complexes and their Antibacterial Efficiency

Abstract

The objective of the present study was to prepare, characterize silver nanoparticles /antibiotics complexes and examine their antibacterial activities. Silver nanoparticles were prepared using chemical reduction method (sodium citrate) and characterized by UV/Vis, FTIR and TEM. The results have shown the characteristic UV absorption at 415 nm. Furthermore, TEM analysis demonstrated the presence of spherical, rod and hexagonal of silver nanoparticles. The infrared spectrum of Clotrimazole exhibited a characteristic peak of C= N at 1594 cm−1, which showed significant shifts to 1585 cm−1 in the infrared spectra of the Clotrimazole+AgNPs complexes, which confirmed the participation of the C= N group in the complexation process The infrared spectrum of Tinidazole exhibited a characteristic peak of C=N at 1760 cm−1, which showed significant shifts to 1621 cm−1in the infrared spectra of the Tinidazole+Ag NPs complexes, which confirmed the participation of the C= N group in the complexation process. A comparison of the relevant IR spectral bands of clotrimazole and tindiazole with the silver nanoparticle indicated shifts in the frequencies as well as reductions in the band intensities, which proved the formation of charge transfer complexes. These shifts were due to changes in the electronic structures and molecular symmetries of reactants upon complex formation. The Antibacterial activity of Ag nanoparticles alone and in combination with the antibiotics such as clotrimazole and tindiazole against Staph. aureus , Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa, showed a significant increase in antibacterial activity of antibiotics in the presence of v silver nanoparticles, a high synergistic activity of nanoparticles with clotrimazole against Staph. Aureus was observed.