Study of Genetic Variability, Quality Parameters, and Molecular Characterization in some Rice (Oryza sativa L.) Genotypes

Abstract

This experiment was conducted in two locations, Experimental farm of College of Agricultural Studies (Shambat), Sudan university of Science and Technology in the growing season of (2011 and 2013) and, the second location was the experimental farm University of Bakht Alruda (ED duiam) at the growing season 2012. In order to investigate genetic variability, quality parameters and molecular characterization in 18 rice genotypes. Data were recorded on growth traits yield traits. Results for the analysis of individual variation detected significant difference among the tested genotypes for most of the studied traits in all seasons. Combine analysis, showed significant differences for genotypes and interaction of genotype X season. The best yielding genotypes were Handao221 (4.03 t/ha) for the year 2011 and Nerica14 of 2012 (3.50 t/ha) and Yunlu33 -of season 2013 (2.43 t/ha), the best yielding genotype for combine analysis was Zhonghan3 (2.38 t/ha). The seeds were taken to the laboratory for quality information which is percentage of a physic-chemical characteristics, minerals profile (Ca, P, Fe, Zn, Mn, Cu) and Physical prosperities (color, granule size, and taste) the results showed that Yulus genotypes were the best in quality while Nericas genotypes were the best content of protein .The three primers OPK16, OPL18 and OPG05 showed the average percentage of Polymorphic bands was 89.53%. Cluster analysis grouped the 18 genotypes into 2 distinct main clusters, and 5 sup cluster