Detection of- β lactamases Wide Spectrum Among Bacteria Isolated from Patients in Military Hospital, Omdurman

Abstract

The emergences of extended-spectrum beta-lactamases (ESBLs) producing bacteria have important clinical and therapeutic implications. The present study was undertaken to detect extended spectrum betalactamases among bacteria isolated from different clinical specimens. This study was carried out during period from April to August 2018. A total of 100 specimens were collected from patients admitted to Omdurman Military Hospital. These were from urine 73(73%), wound swab 20(20%) and sputum 7(7%). The specimens were collected from both males 49 (49%) and females 51 (51%). The ages of participants were range from3-88years. The specimens were cultured on MacConkey agar, CLED agar and blood agar. The isolates were identified by their colonial morphology, Gram stain and biochemical tests. Identified bacteria were subjected to antimicrobial sensitivity test. The antibiotics used were: Gentamicin, Imepnem, Cipofloxcin, Ceftazidime, Amikacin and Norofloxacin. The presence of ESBLs production was detected by double disk synergy and combination disc tests. The results revealed that 65(65%) of the specimens gave bacterial growth, while 35(35%) showed no bacterial growth. 38(58.5%) of bacteria were isolated from urine, 20 (30.8%) from wound swabs and 7 (10.8%) from sputum. The identified bacterial isolates were K. pneumoniae 28 (43.1%), VI E. coli 27 (41.5%), P. aeruginosa 7 (10.8%), and Proteus spp 3 (4.6%). ESBLs were detected in 12(18.5%) bacterial isolates. These were 11(16.9%) E. coli and 1(1.5%) K. pneumoniae. The positive ESBLs producers 5(7.7%) were obtained from males and 7(10.8%) from females. The frequency of ESBLs producers among the groups were2(3.1%) in youth 4(6.2%) in adults and 6 (9.2%) in elderly, 4(6.2%). The study concluded that there is high ratio of ESBLs among isolated bacteria in Military Hospital. The most ESBLs producers are E. coli. Further studies using large number of specimens and advanced techniques are required to validate the result of this study.