Immunohistochemical Detection of Survivin in Non-Hodgkin᾿s Lymphoma among Sudanese Patients

Abstract

This is an analytical retrospective case control hospital based study was conducted in Radiation and Isotope Center Khartoum (RICK), during the period from August 2016 to February 2017. The study aimed to detect Survivin expression in non-Hodgkin᾿s lymphoma among Sudanese patients using immunohistochemistry. Forty paraffin embedded blocks from patient’s samples previously diagnosed as non-Hodgkin᾿s lymphoma were collected. Samples include 26 (65%) malignant tumors, (including diffuse large B.cell lymphoma 17 (42.5%) samples, Burkhits lymphoma 2 (5.0%) samples, anablastic large cell lymphoma in 1 (2.5%) samples, B.cell non- Hodgkin᾿s lymphoma 2 (5%) samples, follicular lymphoma 2 (5%) samples, small lymphocytic lymphoma 2 (5%) samples) and 14 (35%) reactive hyperplasia benign samples. The patient’s age ranged between 22 and 65 years with mean age of 45 years, most patients were above 40 years representing 28 (70%) and the remaining 12 (30%) patients were under 40 years. One section of 3 micrometer thickness was cut from each paraffin block by rotary microtome and stained by immunohistochemical method (indirect streptoavidin-biotin immunoperoxidase technique) for detection of Survivin. Data collected from patients files and results obtained were analyzed using SPSS computer program. Immunohistochemical expression of survivin was revealed positive expression in 20/26 samples and negative expression in 6/26 samples in malignant, while benign samples revealed positive expression in 4/14 samples and negative expression in 10/14 samples for survivin with significant statistical association between survivin expression and histopathology diagnosis of non-Hodgkin᾿s lymphoma (P.value = 0.006). This study concludes that Survivin expression is associated with malignant forms of non-Hodgkin᾿s lymphoma.