Antibiotic Resistant Microflora Bacteria in Intestine of Broiler Chicken

Abstract

This study was done to investigate antibiotic resistance of micro flora isolated from cloacal swabs ofhealthy broiler chicken aged 57-60 day to 9 different antibiotics. A total of 50 cloacal swabs was collected from two farms in Omdurman county(Khartoum state) 72 aerobic bacteria were isolated, 15(21%) were Gram positive while the remaining 57(79%) were Gram negative bacteria. Susceptibility test were done using disc diffusion method for the isolated bacteria .Only 4 Gram negative isolates were found sensitive to all antibiotics used ,the remaining 53 isolates showed different resistance to antibiotic.Higher resistance was observed in cefapime while higher sensitivity was detected in Gentamycin. Gram positive bacteria showed different resistancepattern toantibiotics. Higher resistance was observed to cefapime and erythromycin while higher sensitivity was detected to colistin, gentamycin and amoxicillin.Multi resistance pattern was detected in 34 (47%) of isolated bacteria. Regarding antibiotics higher sensitivity was found to colistin (75%) followed by gentamicin (53%) and ciprofloxacin (45%) while only (9%) of the isolates showed sensitivity to cefapime. Higher resistance was detected to cefapime (76%) followed by doxycycline (53%) and tetracycline (49%) while 7 (10%) isolates were found resistant to colistin