Effect of Age on Success Rate of IVF Treatment among Infertile Sudanese women In Khartoum state

Abstract

Infertility is the most widespread and considered as one of the most important unappreciated health problems, particularly in developing countries and about 10% of women failure to achieve a normal pregnancy. This study was conducted to estimate plasma FSH, LH and PRL among infertile Sudanese women. The study included one hundred and fifty women participate in this study and divided into three groups according to the age of participant, group (1) (35-37 ) years included 44 women, group (2) (38-40) years included 59 women and group (3) (41-45)years included 47 women. One hundred and fifty blood samples were collected from these women during period from January to July 2017, chosen from Sudan Assisted Reproduction Center (SARC). Fluorescence immunoassay (FIA) method used to estimate plasma FSH, LH and PRL concentration, and results were analyzed using statistical package for social science (SPSS) computer program. The study showed that, the means concentration of FSH were significantly increase in women group (2) and group (3) compared to group (1) of infertile Sudanese women . Mean ±SD :(10.32±7.03 versus 7.98±3.36 mIU|ml ,p. value=0.03 ). (11.19±6.40 versus 7.98 ± 3.36mIU|ml, p -value=0 .007) respectively, while there was insignificant different in FSH level between group (3) compared to group (2) (11.19±6.40 versus 10.32 ± 7.03mIU|ml ,p- value = 0.5). Result of this study showed, there were insignificant different between the means of LH and prolactin levels when compared between study groups ( p- value≥0.05). Mean ±SD for group3 versus group2: (5.06±2.74 versus 5.46 ± 2.95mIU|ml) for LH, (22.62±16.05 versus 21.45 ± 9.70ng|ml) for PRL.group2 versus group1: (5.46 ±2.95 versus 5.30±2.24mIU|ml) for LH. (21.45 ±16.05 versus 19.94±12.92 ng|ml) for PRL . group3 versus group1:(5.06±2.74 versus 5.30 ± 2.24mIU|ml) for LH. (22.62±16.05 versus 19.94 ± 12.92ng|ml) for PRL. Other factors shared age to increase risk of infertility in Sudanese women included: female factor ( tubal block 20.6%, PCO 12% and fibroid 1.3%) , male factor (34.7%), both male and female factors (6.7%)and un explaining factor (24.7%). The study also showed there was significant weak positive correlation between age and FSH concentration . (r =0.198, P. Value = 0.01), while there was no correlation between LH and age .(r = 0.016,P. Value = 0.84).The study showed there was insignificant weak negative correlation between age and PRL . (r= - 0.19, P .Value=0.23). The success rate of IVF treatment,21.3% ofinfertile women under IVF treatment have positive result (β -HCG)(10.6% in group (1) ,8.67% in group (2) and 2.0% in group (3) ).while 78.7% of infertile women under IVF treatment have negative result (β -HCG). It is concluded that the plasma level of FSH was significantly increase in infertile Sudanese women , while the plasma levels of LH and PRL showed insignificant different, also there was significant positive correlation between FSH and age, and success rate of IVF decrease with age.