Comparative of The Allo-antibodies in Repeated Blood Transfusion Recipient Using Conventional Tube Method and GelMethod

Abstract

Repeated blood transfusion is important for patients as basic therapy, it is used to treat and prevent the complications of many diseases. The most serious complications are haemolytic transfusion reactions in which there is increased red cell destruction which caused by repeatedblood transfusion, allergic reactions and febrile reactions which are due to antigen antibody reaction. The aim of this study is to compare between the two different protocols; conventional tube method and ID cardmicrotyping system, to detect the frequencyof antibodies in repeated blood transfusion. This is an analyticalcomparative study in which193 patients with repeated blood transfusion were enrolled. Complete blood count was done using (Sysmex) 21.Then sickling test, hemoglobin electrophoresis at alkaline pH (8.4–8.6), and antibody screening with conventional tube andgel methods were done. The results were analyzed. Antibodies screening using conventional method showed that 3 patients were positive,190 patients were negative, while gel method showed that 5 patients were positive, while188 patients were negative with repeated transfusion.The study Concluded that thedifference the between two methods was present,conventional tube andgel method withdifferent P-value (0.22-0.41),respectively, it was insignificant. Gel method is more accurate and rapid, appropriate for detection of antibodies, and more accurate, to facilitate selection of blood,