Assessment of Estrogen and Progesterone Level in the Dromedary Camels after Ovulation Induction during Non-Breading Season

Abstract

Abstract The aim of the present study was to determine the serum Estrogen and Progesterone concentrations after ovulation induction using GnRH and hCG in the dromedary camel. A total number of (19) dromedary camels used in this experiment was divided into three groups during the non-breeding season: Group A (N=7), animals were intramuscular injected by 2 ml GnRH, group B (N=6), animals were intravenous injected by 3 ml hCG. Group C (N=6) control group, animals were intramuscular injected by 1 ml distilled water. Blood samples were collected at alternate days throughout the defined experimental periods (0 hours = time of injections, 48 hours = after 48 hours, Week 1 = after 7 days, and Week 2 = after 14 days), to determine estrogen and progesterone levels using ELISA. Results showed that serum estrogen concentrations (pg / ml) did not differ significantly (P ? 0.05) between the all groups in 48 hours and week 2. In contrast in 0 hours and week 1 which were higher significant (P ? 0.05) between the treated groups (GnRH and hCG groups) compared with control group, while there was no significant difference between the treated groups throughout the alternate periods. Serum progesterone concentrations (ng / dl) did not differ significantly (P ? 0.05) among the all groups in 0 hours and week 2. The serum progesterone concentration in 48 hours were significant higher (P ? 0.01) in the (A) group compared to the (B) and the control groups respectively. Also the levels of hormone were higher significant (P ? 0.01) in the (B) group compared to the (A) and control groups respectively. It can be concluded that the hormonal analysis of estrogen and progesterone in case of follicular ovarian wave indicated a large individual variation.