Detection of β- Lactamases Genes in some Salmonella Isolated from Poultry in Khartoum North, Sudan

Abstract

Abstract The occurrence of extended spectrum beta lactamases genes in bacteria (ESBLs) is one of the problems that facing the world now in treatment of bacterial infection. This study was conducted to detect CTX-M, SHV, and TEM genes in ESBLs producing Salmonella gallinarum and Salmonella pullorum. All Salmonella strains were isolated from samples collected from poultry farms located in Khartoum north and identified with conventional methods. Bacterial DNA was extracted from each isolate (S.pullorum, S.gallinarum) using boiling method. PCR was used to detect TEM, SHV, and CTX-M genes. The results showed that the genotypic resistance that is mediated by ?-lactamases genes in S. gallinarum was (100%) for SHV followed by CTX-M and TEM genes both (58%) and in S. pullorum was (44%) for CTX-M then TEM (33%) and finally SHV genes (11%).