ANTIMICROBIAL ACTIVITY AND MINIMUM INHIBITION CONCENTRATION (MIC) OF ACACIA NILOTICA EXTRACTED BY USING ACETONE, CHLOROFORM, ETHANOL, AND METHANOL SOLVENTS

Abstract

This research studied the Acacia nilotica fruits (named Garad in Sudan) extract as antimicrobial agent. Samples were well prepared by garbling in powder form, extracted by solvent solution using acetone, chloroform, ethanol, and methanol, Acacia nilotica (fruit) extract was punched on the Erechie - coil and a Staphylococcus aureus organism which often found on the wound skin. The zone area of inhibition in both E. coli and S. aureus, was best found to be determined, methanolic extract showing the highest activity and more significant to inhibit the organisms compared to the other solvents herbal extract.The minimum inhibition concentration(MIC) is also determined at varying concentrations and it is clear that MIC result is good and fall between 40 &100mg/ml for E. coli , 50 & 100 mg/ml for S. aurous . GC –MS analysis was done for the herbal extracts in order to confirm the presence of the bioactive components