Identification of Bacterial Pathogens Isolated from Haemodialysis Patients using VITEK 2 Compact System and their Antibiotic Susceptibility

Abstract

This study was conducted to investigate catheter-related bloodstream infection (CRBI) of 201 chronic haemodialysis (HD) patients with temporary, or true permanent access (catheters) (PCs) who had fever, chills, or other symptoms suggesting systemic infection attended 17 dialysis units in Khartoum State during 12 month period. Blood infection is often the reason for hospitalization and the cause of mortality across the globe. In this study, patients with HD CRBSIs were identified. Their blood cultures were collected according to standard sterile technique. Specimens were sent to the microbiology lab for culture and primary identification of colonial morphology and Gram-stain. Isolates were then run on the semi automated Vitek 2 Compact System (bioM�rieux, France). Sixty two patients ranged in age between 10 and 90 years were confirmed to have HD CRBSIs based on our study criteria. Fifty six (90.3%) patients had Gram-positive infections and six (9.7%) patients had Gram-negative infections. The antibiotic susceptibility results showed that only vancomycin, linezolid, tigecycline and nitrofuranation were fully efficacious against Gram-positive isolates, and were highly resistant to benzylpenicillin (92.9%) and oxacillin (83.9%). Susceptibility results of Gram-negative isolates showed fully resistance (100%) to ampicillin, ampicillin/sulbactam, cefazolin and cefoxitin and all isolates were susceptible to amikacin. Our study revealed that Staphylococcus epidermidis was the most common microorganism associated with HD CRBSIs. Antibiogram is an important tool in deciding empirical antibiotics for HD CRBSIs. Tailoring the antibiotics accordingly to the antibiogram can increase the chance of successful treatment and prevent the emergence of bacterial resistance