Fabrication of an Immunochromatography Biosensor Using Green Synthesis Gold Nanoparticles

Abstract

An Immunochromatography (ICT) bio-detector facilitates low-cost, rapid identification of various analytes at the point of care. The ICT cell consists of a porous membrane containing immobilized ligands at various locations. Through the action of capillary forces, samples and reporter particles are transported to the ligand site. A mathematical model for assays is constructed and used to study the performance of the ICT under various operating concentration of particles and analytes. The model provides insights into certain experimental observations including the reduction in the level of the detected signal and the contrast .The model can be used to test rapidly and inexpensively various operating conditions assist in the device's design. The research is focused on green synthesis methods to synthesis gold nanoparticles using cheap and nontoxic chemicals, ecofriendly and safer then study the particle size and concentration for capability to be used in the devices design .From TEM results the sizes of particles are 15 and 20 nm that mean can be used for sensing.