Detection of CDX2 Tumor Marker and Epstein Barr Virus among Sudanese Esophageal Tumors Patients by using Immunohistochemistry

Abstract

This is a descriptive retrospective study carried out in IbnSenaHospital and Sudan University of Science and Technology, college of Medical Laboratory Science during the period between April 2014 to October2014.The study aimed at detection of CDX2 and EBV immunohistochemically in esophageal tumors among Sudanese patients. 30 paraffin blocks previously diagnosed as esophageal tumors, 20 were diagnosed as esophageal cancer and 10 were diagnosed as benign esophageal tumors, were used in this study ages ranging from 8– 98 years with mean age of 59 years old. Two sections were taken from paraffin wax embedded blocks and placed in coated slide to detect CDX2 and EBVimmunohistochemically. SPSS version 11.5 computer program was used to analyze the data, frequencies; means and Chi squire test were calculated. Out of 30 patients with esophageal tumor, 20(66.7%) patients were males and 10(33.3%) were females with ratio 1:2. Out of30 samples 4(13.3%) cases positive for CDX2 in malignant sample and 26(86.7%) cases were negative with no relation between CDX2 expression and type of tumors (P.value:0.129). Out of 30 samples 5(16.7%) cases were positivefor EBV, while 25(83.3%) cases were negative with no relation between EBV infection and type of tumors (P.value: 0.083). On the basis of These finding the study concluded that there is no association between CDX2 expression and EBV infection with type of esophageal tumors.