Bacterial Contamination in Surgery Theaters in Hospitals of Khartoum State - Sudan

Abstract

The study was conducted during the period of August to October 2012 in Khartoum state private hospitals (Khartoum, Bahri and Umdurman). During the course of this study a total 460 samples from 46 private hospitals and centers were collected. 25 hospitals and centers have been detected as contaminated with a rate of (54.3%). The Specimens were collected from 10 sites of operation room’s environment. The positive samples were 40 (8.69%) from whole collected samples and 9 types of bacteria were isolated from the contaminated hospitals. Contamination ratio was (72.2%), (58.3%) and (31.3%) in Khartoum, Umdurman and Bahri respectively. The growth culture was 10.9% in Khartoum, 11% in Umdurman and 4.7% in Bahri. Gram-positive bacteria represent 11(27.5%) of the total isolates, while Gram-negative bacteria represent 29(72.5%), the predominant isolated organism were pseudomonas aeruginosa (32.5%) ,Cedecea (20%), Staphylococcus aureus (15%), Enterobacter cloacae (10%), Staphylococcus Epidermidis (10%) Escherichia coli (5%), Enterobacter aerogenes, Bacillus subtilis and Citrobacter freundii was at the same level (2.5%). The highest contaminated area in operation rooms site were the sucker (37.5%) followed by Trolley(35%), focusing lamp(7.5%), Oxygen cylinder( 5%), floors, anesthesia mask, washing basin, child bed, cauterize machine and cupboard were at the same percentage (2.5%).