Polymorphisms in Glutathione S-Transferees (GST) M1 and S-Transferees (GST) T1 Genes in Sudanese Patients with Myeloid Leukemia

Abstract

This study was done in Radiation Iso top centre during the period 2011 to 2012 to assess the role of the Gene deletion of the detoxification enzymes glutathione S- transferees GSTMI and GSTTI in the occurrence of myeloid leukemia in Sudan. The study included 30 patients with acute myeloid leukemia, 30 patients with chronic leukemia and 30 healthy controls. According to this study (46.7%) of the CML patients were from Khartoum State (where RICK) is located followed by the western Sudan in case of CML. (40%) of the AML patients were between the age of (40-50) years on the other hand most of the CML patients were between (40-50) the controls were between 31 - 40 years old. The study revealed than both AML and CML patients were mostly from the Afro-asiatic population group, followed by Nile-Saharan in case of AML. In this study the male / female ratio of AML was (1:2) (36.3% - 63.7%) while that of CML was (2:1) (66.7%-33.3%), the control group included males and females male / female ratio is (43.3%-56.7%). Laboratory Methods: 1- Salting out method (DNA extraction). 2- Electrophoresis of extracted DNA. 3- Stander of PCR reaction (next Generation of premix (Germany CAPS. ALHP) master- mix. Genotyping of patients showed that only 2 of the AML patients, 7 of the CML patients and 8 controls showed GSTMI while 22 of the AML patients 23 of the CML patients and 11 controls showed detected for the GSTTI. For deletion in GSTM1 showed 6.67% of AML was deleted and, 23% of CML and 26.67% of controls were detected on the other hand for GSTTI and only 73% of AML 76.67% of CML and only 36.67% of controls were detected.