SUST Repository

ASSESSMENT OF THE POTENTIAL EFFICACY OF BEE VENOM AS AN ANTILEISHMANIAL CHEMOTHERAPEUTIC (IN VITRO)

Show simple item record

dc.contributor.author IBRAHIM, HOYAM MAHGOUB
dc.contributor.author Supervisor,- ADIL M. IBRAHIM
dc.date.accessioned 2014-06-11T06:53:05Z
dc.date.available 2014-06-11T06:53:05Z
dc.date.issued 2005-01-01
dc.identifier.citation IBRAHIM,HOYAM MAHGOUB.ASSESSMENT OF THE POTENTIAL EFFICACY OF BEE VENOM AS AN ANTILEISHMANIAL CHEMOTHERAPEUTIC-IN VITRO/HOYAM MAHGOUB IBRAHIM;ADIL M. IBRAHIM.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2005.-56p. : ill. ; 28cm.-M.Sc. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/5494
dc.description Thesis en_US
dc.description.abstract This study essentially evaluated the antileishmanial activity and toxicity of the bee venom using in vitro techniques. The study also compared in vitro the maintenance of L.donovani promastigotes in 2 types of culture media, 3N and Hamin- agar media .The preliminary in vitro examination of the potential antileishmanial effect of bee venom upon L. donovani promastigotes when treated with 10-fold dilutions of this chemotherapeutic agent (175X10-3- 0.0017X10-3μg/ml) for 24h at 25Co. The highest response of growth inhibition (>70%) was obtained when the parasite subjected to bee venom concentrations >1.0X10-3μg/ml but a total inhibition of parasite population growth was only recorded at concentration 175X10-3μg/ml. IC50 was calculated as 3.0μg/ml. The lymphocyte proliferation assay for toxicity was used to compare the bee venom toxicity with Pentostam (Wellcome, UK), a standard antileishmanial drug. The cytotoxic effect of bee venom has been expressed as the mean percent inhibition of cell proliferation after 48h treatment with bee venom. About 40% inhibition of lymphocyte proliferation was observed when the lymphocytes were subjected in vitro to the highest concentration of this therapeutic agent (175X10-3μg/ml) , Pentostam had no effect even at maximum tested concentration 500μg/ml whereas bee venom appeared to have some toxicity at concentrations <0.1X10-3μg/ml only. On the other hand treatment of PMBC with low concentrations (0.001-0.1X10-3μg/ml) of bee venom significantly stimulated PMBC proliferation (P<0.05). en_US
dc.description.sponsorship SUDAN UNIVERSITY OF SCIENCE & TECHNOLOGY en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science and Technology en_US
dc.subject POTENTIAL EFFICACY-BEE VENOM-ANTILEISHMANIAL en_US
dc.subject CHEMOTHERAPEUTIC en_US
dc.title ASSESSMENT OF THE POTENTIAL EFFICACY OF BEE VENOM AS AN ANTILEISHMANIAL CHEMOTHERAPEUTIC (IN VITRO) en_US
dc.type Thesis en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Share

Search DSpace


Browse

My Account