Abstract:
This study was carried out in Khartoum State in period November 2008 to
March 200, to determine the frequency and resistance of Salmonella
paratyphi A isolated from patients suspected to have enteric fever. A total
of 308 stool samples were collected from Khartoum Teaching Hospital,
Umderman Teaching Hospital, Khartoum North Hospital, and Ibrahim
Malik Teaching Hospital. The age of patients ranged from one year to 80
years. The samples were cultured on Xylose Lysine Deoxycholate agar
for primary isolation of pathogen. Identification of the isolates was
done by colonial morphology, Gram stain, oxidase test and biochemical
tests using API E20.Resistance to antimicrobial agents were determined by
modified Kirby- Bauer diffution method while MIC was determined by E –
test.
Only 11 (5.6%) Salmonella paratyphi A from 308 stool samples, were
detected. The result revealed
that the resistance rate of
antimicrobial
agents against the isolates was as follows; (9.1%) of Choloramphincol and
Ceftrixone,
(81.1%)
of
Tetracycline,
(0%)
of
Ciproflaxcine
and
Gentamycine, (45.5%) of Amoxycilline, (36.4%) of Co-trimoxzol, and
(100%) of Ceftazedine .
The minimum inhibitory concentration values were ( 0.01 - 1.0 mcg/ml ) of
Ciproflaxcine, ( 0.004 – 0.1 mcg/ml ) for Choloramphincol, and (10 – 60
mcg/ml) for Tetracycline.
The study concluded that the responsibility of Sallmonella paratyphi A
of enteric fever is slightly low. The antimicrobials resistance of S.
paratyphi A to traditionally used antimicrobial agents was high.
