dc.contributor.author |
Khalafalla, Nosaiba Mohammed Alamin |
|
dc.contributor.author |
Supervisor,- Yousif Fadlalla HamedElnil |
|
dc.date.accessioned |
2014-03-19T08:52:13Z |
|
dc.date.available |
2014-03-19T08:52:13Z |
|
dc.date.issued |
2013-09-01 |
|
dc.identifier.citation |
Khalafalla,Nosaiba Mohammed Alamin.Detection of Extended–spectrum Beta Lactamase Genes in Urinary Tract Isolated Bacteria among Pregnant Women/Nosaiba Mohammed Alamin Khalafalla;Yousif Fadlalla HamedElnil.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2013.-48p. : ill. ; 28cm.-M.Sc. |
en_US |
dc.identifier.uri |
http://repository.sustech.edu/handle/123456789/3993 |
|
dc.description |
Thesis |
en_US |
dc.description.abstract |
The aim of this study was to detect extended spectrum β-lactamase (ESBL)
in bacterial isolated from urine collected from urinary tract infected pregnant
women. A total of 100 midstream urine samples were collected from
Omdurman Militory Hospital during April 2013. Specimens were cultured
on CLED agar to identify UTI causative bacteria by colonial morphology,
Gram stain and routine biochemical tests. Out of 100 urine specimen;
56(56%) specimen gave significant bacterial growth, the Grame negative
bacilli were 42(75%) and 14(25%) Gram positive. The most frequenty Gram
negative was K. oxytoca 15 (26.8%) and E. coli 10(17.9%), while the most
abundant Gram positive was S. saprophyticus. The susceptibility testing, 5
isolates were resistant to 3rd generation cephalosporines (ceftazidime,
ceftriaone and cefotaxime) as follow: 2 K. pneumoniae ,2 E. coli and 1 P.
vulgaris.
ESBL production test done for all bacterial resistance to 3rd
generation cephalosporin and then confirmed by combination test by using
Double disc synergy test 5(11.9% ) isolates gave positive result. The PCR
was done for 5 isolates using TEM, SHV, and CTX-M primers, TEM gene
was the most predominant one 5(100%) followed by CTX-M 3(60% )and
the least one was SHV gene 2(40% ). |
en_US |
dc.description.sponsorship |
Sudan University of Science and Technology |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Sudan University of Science and Technology |
en_US |
dc.subject |
Beta Lactamase Genes |
en_US |
dc.subject |
Urinary Tract-Bacteria-Pregnant Women |
en_US |
dc.title |
Detection of Extended–spectrum Beta Lactamase Genes in Urinary Tract Isolated Bacteria among Pregnant Women |
en_US |
dc.type |
Thesis |
en_US |