Abstract:
This study was conducted in the plant tissue culture laboratory of the
College of Agricultural Studies, Sudan University of Science and
Technology. The study aimed to fulfill the following objectives :-
Exploration of the different substances as disinfectants to establish the in
vitro culture of ginger shoot bud explants; investigation of the influence of
different furidan concentrations on cultures multiplication and exploration of
the effect of different media physical forms on proliferation of in vitro
cultures of ginger.
The results showed variations in the capacities of the various disinfectants
to control microbial infections of the explants. The water extract of willow
bark exhibited the best potential for control of both fungal and bacterial
infections. An antibiotic (Gentamicin) was also of considerable control
powers for both bacterial and fungal microbes. The best antifungal activity
was obtained from the from the milky latex of the leaves and stems of Gaint
milk weed (Calotropis procera).
The concentration 2mg/l carbofuran (furidan) enhanced the multiplication
potential of cultures.
The agar mounted by a layer of liquid media was better, although not
significantly different from agar media for shoot multiplication, while
multiplication was retarded in stationary liquid media.