Abstract:
This study was carried out in the period from May 2007- March 2008 to detect the
presence of Hafnia alvei in patients suffering from urinary tract infection. Forty six
urine specimens were collected from patients attended Khartoum Teaching Hospital,
Ibrahim Malik Teaching Hospital and Omdurman Teaching Hospital. Bacterial DNA
was extracted from each urine specimen using Phenol-Choloroform technique. Real
time PCR technique was adopted to detect the presence of Hafnia alvei.
The results revealed that only one (2.1%) specimen was positive and the rest forty
five (97.8%) were negative. The study concluded that real time PCR technique
facilitates detection of Hafnia alvei in urine without bacteriological culture.