Abstract:
This study was carried out in Khartoum state during the period
between May 2007_ March 2008 to detect the presence of S. typhi in
patients with suspected enteric fever. Forty-seven blood specimens
were collected from patient at three hospitals: Khartoum Teaching
Hospital, 14 (29%), Omdurman Teaching Hospitals, 28(59%) and
Alengath Medical Center 6 (12%). S.typhi DNA was extracted directly
from each blood specimens using phenol - chloroform technique.
Real time PCR technique was adopted to detect presence of S. typhi.
The result revealed that only 7 (15%) specimens were positive and
40 (85%) were negative.
The study concluded that the real time PCR technique facilitates
detection of S.typhi in blood of patients with suspected typhoid fever
without bacteriological culture.
