Abstract:
This study was carried out in Khartoum state during the period between
March 2007_ March 2008 to detect the presence of S. pratyphi A in patients
with enteric fever. Forty-six blood specimens were collected from patients
attended tow three hospitals: Khartoum Teaching Hospital, 26 (56.5%),
Omdurman Teaching Hospitals, 12(26.1%) and Gaffer Ibnouf Specialized
Hospital 8 (17.4%).
S. pratyphi A DNA was extracted directly from each blood specimens using
phenol - chloroform technique. Real time PCR technique was adopted to
detect presence of S. pratyphi A The result revealed that only 2 (4.3%)
specimens were positive and 44 (95.7%) were negative.
The study concluded that the real time PCR technique facilitates detection
of S. pratyphi A in blood of patients with without bacteriological culture.