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Detection of Enterobacter cloacae in Stool Specimens by Real-time Polymerase Chain Reaction

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dc.contributor.author Ahmed, Elnaim Bushra
dc.contributor.author Supervisor,- Humodi Ahmed Saeed
dc.date.accessioned 2014-02-05T12:33:36Z
dc.date.available 2014-02-05T12:33:36Z
dc.date.issued 2008-01-01
dc.identifier.citation Ahmed,Elnaim Bushra.Detection of Enterobacter cloacae in Stool Specimens by Real-time Polymerase Chain Reaction/Elnaim Bushra Ahmed;Humodi Ahmed Saeed.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2008.-36p. : ill. ; 28cm.-M.Sc. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/3382
dc.description Thesis en_US
dc.description.abstract This study was carried out during the period from May 2007 to March 2008 to detect the presence of Enterobacter cloacae in stool specimens from patients suffering from gastrointestinal disturbance. Forty six samples were collected from patients attended to Khartoum Teaching Hospital, Omdurman Teaching Hospital, Khartoum pediatrics Hospital and Omdurman pediatrics Hospital. DNA was extracted directly from stool specimens and used for the subsequent experiment. The forty six specimens were examined using Real time PCR Technique. The study revealed only seven (15c/o) specimens were positive and the remained specimens thirty nine (85%) were negative. The study concluded that the real-time PCR technique facilitates rapid detection of .bacterial pathogens directly from clinical specimen without culturing of bacteria en_US
dc.description.sponsorship Sudan University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science and Technology en_US
dc.subject Enterobacter cloacae en_US
dc.subject Stool-Specimens en_US
dc.subject Microbiology en_US
dc.subject gastrointestinal disturbance en_US
dc.title Detection of Enterobacter cloacae in Stool Specimens by Real-time Polymerase Chain Reaction en_US
dc.title.alternative ‫كشف الامعائية المزرقية فى عينات البراز بتقنية تفاعل البلمرة المتسلسل‬ ‫الزمني‬ en_US
dc.type Thesis en_US


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