Abstract:
This study was carried out in the period from May 2007- March 2008 to
detect the presence of Salmonella enteritidis in patients suffering from
gastroenteritis. Forty-seven stool specimens were collected from patients
attending Albangadid teaching hospital and Alzahra medical centre.
Bacterial DNA was extracted from each stool specimen using phenol
choloroform technique.
Real-time PCR technique was adopted to detect presence of Salmonella
enteritidis.
The result revealed that only one (2.1%) specimen was positive.
The study concluded that the Real-time PCR technique facilitates
detection of bacterial pathogens without bacteriological culture.