Abstract:
This study was carried out during the period from May 2007- March 2008 to detect
the presence of Edwardsiella tarda in patients with diarrheal disease. Forty six stool
specimens were collected from patients attended Omdurman Teaching Hospital,
Bashair Teaching Hospital and Jafar Ibn ouf Pediatrics' Hospitals. Bacterial DNA was
extracted from each stool specimen using phenol –chloroform technique.
Quantica thermcycler was used to detect Edwardsiella tarda. The result revealed that
only 2 (4.3%) specimens were positive and 44 (95.7%) were negative. The study
concluded that the real time PCR technique facilitates detection of E. tarda pathogens.