Abstract:
This study was carried out in the period from May 2007 - March 2008 to detect the
presence of Serratia marcescens in patients suffering from pneumonia. Forty six sputum
specimens were collected from patients who attended Albangadid Hospital, Alshaab
.Hospital and National Health Laboratory
Bacterial DNA was extracted from each sputum specimen using phenol chloroform
method. Real Time PCR technique was adopted to detect the presence of Serratia
.marcescens
The result revealed that only three (6.5%) specimens were positive and the rest (93.4%)
. were negative
The study concluded that the Real-time PCR technique facilitates detection of bacterial
.pathogens without the need for bacteriological culture