Abstract:
This study was carried out in the period from May 2007 - March 2008 to detect the
presence of Klebsiella pneumoniae in patients suffering from pneumonia. Forty seven
sputum samples were collected from patients attended Albangadid Hospital, Alshab
Hospital, and National Health Laboratory.
Bacterial DNA was extracted from each sputum specimen using Phenol Chloroform
method. Real-time PCR technique was adopted to detect the presence of Klebsiella
pneumoniae using Real Time PCR.
The result revealed that only three (6.3%) specimens were positive.
The study concluded that the gene probe technique facilitates detection of bacterial
pathogens without bacteriological culture.
