Abstract:
This is analytical case control study carried out in Khartoum
state during the period from November 2008-March 2009.
The study aimed to asses the cytomorphological pattern of
sputum in cigarettes smokers using conventional cytological stain
(papanicolaou stain) in addition to the evaluation of the role of Ag
NORs in the identification of cytological atypia using silver-nitrate
method.
In this study 50 sputum samples from male cigarette smokers
were collected as study group, the duration of smoking was 15 years
or more, and the number of cigarette smoked is more than 10 cigarette
per day, and they were without respiratory tract infections and
previous history of lung diseases as test, and 50 samples of sputum
from non smokers as control, their age between 22 and 65 years.
Two smears were prepared from each specimen then stained using
two different technique papanicolaou stain &silver-nitrate.
Inflammatory cells were detected in both group as 46% acute
inflammation and 4%chronic
inflammation from smokers group, in
non smokers acute Inflammation were detected in 1%, 3%chronic
inflammation, with significantly no correlation in the severity of
inflammation, and the smoking duration, and number of cigarette per
day.
Metaplastic changes were observed in 31%, with a significant
correlation to the duration of smoking and number of cigarette per day
in test group.
Dysplastic changes were detect in 5% and also a significant
correlation to the duration of smoking and number of cigarettes per
day. No malignant cells were detected among the study groups.
Ag NOR showed statistical significant difference between the
mean of Ag NORs in non smokers 2.170 while it was 4.976 in
smoker, and a significant correlation to the number of cigarette per
day but not correlated with duration of smoking.
