Abstract:
This study was carried out in Khartoum state during the
.period between Feb. 2010 and May. 2010
The study aimed to evaluation of the reliability of Anti-
mycolic acid antibody IgM by ELISA method in the
diagnosis of pulmonary tuberculosis compared to ZN
technique and PCR in the diagnosis of pulmonary
.tuberculosis
In this study, ninety (90) sputum and eighty (80) blood
samples were collected from patients suspected to have
.pulmonary tuberculosis
Among enrolled patients, 68 (75.6%) were found males
.and 22 (24.4%) were females
Different
bacteriological,
serological
and
molecular
techniques were conducted. Concerning Ziehl-Neelsen
technique, 17 (18.9%) were positive for acid-fast bacilli
while 73 (81.1%) were negative. At the same time
amplification of IS 6110 reflected 79 (87.8%) positive and
.11(12.2%) negative result
On the other hand, ELISA for anti-mycolic acid anti IgM
showed positive results in 55 (68.75%) and negative in
.(25 (31.25%
From these findings and according to the result obtained
by using the Anti-IgM anti-mycolic acid, the majority
were positive for PCR; 48 (87.1%). However, 7 (12.7%)
were negative to PCR. On the other hand, 45 (81.8%) out
of 55 anti-IgM anti mycolic positive showed negative ZN
smear, while, 10 (8.2%) of them revealed positive result
.by using ZN technique
Additionally, out of 25 negative Anti-IgM sample, 21
(84.0%) were PCR positive and 4 (16.0%) were negative,
while 22 (88%) were negative by ZN stain, but 3 (12%)
.were showed positive results
From these finding, it is concluded that diagnosis of
pulmonary tuberculosis using tedious classical technique
like ZN technique need to be supported by new rapid
technique
like
PCR
and
detection
of
AMA
by
.(serodiagnosis technique (ELISA
