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Molecular Detection of Extended-spectrum Beta-lactamase Genes in Salmonella paratyphi C Isolated from Clinical Specimens

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dc.contributor.author Abdlmageed, Osama Khider Siddig
dc.contributor.author Supervisor,- Humodi Ahmed Saeed
dc.date.accessioned 2013-12-02T07:53:05Z
dc.date.available 2013-12-02T07:53:05Z
dc.date.issued 2010-01-01
dc.identifier.citation Abdlmageed,Osama Khider Siddig.Molecular Detection of Extended-spectrum Beta-lactamase Genes in Salmonella paratyphi C Isolated from Clinical Specimens/Osama Khider Siddig Abdlmageed;Humodi Ahmed Saeed.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2010.-27p. : ill. ; 28cm.-M.Sc. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/2564
dc.description Thesis en_US
dc.description.abstract Extended-spectrum beta-lactamases (ESBLs) is one of the problems that face the world now in the treatment of bacterial infection. This study was conducted in the Research Laboratory in Sudan University of Science and Technology. The study was carried out during the period from December 2009 to May 2010, to detect TEM, SHV and CTX-M genes in ESBLs- producing Salmonella paratyphi C. The Salmonella paratyphi C strains were obtained from the Research Laboratory. All strains were checked for purity by sub- culturing on nutrient agar and examined microscopically. Bacterial DNA was extracted from each isolate using boiling method. Multiplex PCR was used to detect these genes. The result showed the presence of TEM gene only in 33% of the isolates. It is concluded that, TEM is the commonest gene in Salmonella paratyphi C isolates. Further studies with large number of bacterial strains are required to validate this result. en_US
dc.description.sponsorship Sudan University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science and Technology en_US
dc.subject Spectrum Beta en_US
dc.title Molecular Detection of Extended-spectrum Beta-lactamase Genes in Salmonella paratyphi C Isolated from Clinical Specimens en_US
dc.title.alternative الكشف الجزيئي عن جينات انزيمات بيتا لاكتام الممتدة الطيف في السلمونيلة النظيرة التيفية ج
dc.type Thesis en_US


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