Abstract:
This study was conducted in the Research Laboratory in Sudan University of Science
and Technology. The study was carried out during the period from December 2009 to
May 2010, to detect TEM, SHV and CTX-M genes in ESBLs-produsing Salmonella
paratyphi B. The Salmonella paratyphi B strains were obtained from the Research
Laboratory. All strains were checked for purity by sub-culturing on nutrient agar and
examined microscopically. Bacterial DNA was extracted from each isolate using
boiling method. Multiplex PCR was adopted to detect these genes. The result revealed
presence of TEM gene only in Eight of the isolates. It is concluded that, TEM gene is
the commonest gene in Salmonella paratyphi B isolates. Further studies with large
number of bacterial strains are required to validate the present results.