Abstract:
This is an expermental study to assess the preservation of nucleic acids in
three types of tissues by the use of five different fixatives. The study was
conducted in Elobied during the period from April 2010 to august 2010. Blocks
were obtained from rabbit tissue, Formal saline, Buffer Formalin, Carnoy’s,
Methacarn and Elseriow's fixatives were used. Intestinal. Tongue and liver
tissues were taken from a rabbit. Three temperatures of fixation were selected
37°C, 25°C and 4°C.
After application Feulgen and Methyl Green Pyronin for nucleic acids
quantification, Carnoy’s fixative gave high nucleic acids preservation and so was
Methacarn fixative. Elseriow's fixative, which was used for the first time in this
study gave excellant nucleic acids preservation; whereas, Formal saline and
Buffer formalin were gave acceptable results in nucleic acids preservation.
When tissues fixed at 37°C the amount of preserved nucleic acids was
more than these detected at other temperatures, although, results obtained at
25°C was good. Formalin containing fixatives i.e. formal saline and buffer
formalin gave good results at 4°C.
Although, the nucleic acid of all tissue was best preserved, the intestinal
.tissue gave better result followed by liver and lastly the tongue