Abstract:
Diabetes is known caused by relative or absolute insufficiency of insulin
secretion and/or concomitant resistance to the metabolic action of insulin
on target tissues, then hyperglycemias developed. Alpha amylases are
enzymes which hydrolyze starch molecules to give diverse products
including dextrins and progressively smaller polymers composed of
glucose units which cause increase blood glucose. Therefore across
sectional study conduct in Abdoon Seed Ahmed Polyclinic Centre in
Khartoum during the period from April to June 2012 to assess plasma
alpha amylase activity in Sudanese type 2 diabetic patients.5 milliliters of
venous blood was collected from each of 40 females (mean age 40 years)
and 30 males(mean age 51 years). Blood was separated into lithium
heparin container (3mls) for measurement of alpha amylase, glucose and
renal function tests, and EDTA containers for measurement of
HbA1C.Twenty fife apparently healthy volunteers were included as
control 14 males (mean age 51 years) and 11 females (mean age 40
years).
Alpha amylase activity was estimeted by using direct substrate (CNP-
G3).HbA1C was estimated by using Full automated immunoflouresnt
technique, and glucose was estimated by glucooxidase enzymatic method.
Data were analyzed by SPSS computer program. There was insignificant
increase in alpha amylase activity of study group compared to control
group (P value > 0.05) and there was significant deference in study
groups and control groups in fasting plasma glucose and HbA1C (P value
<0.05).
There was no significant difference between diabetic males and diabetic
females regarding alpha amylase, FBG, and HbA1c (P value > 0.05).
It was found that there is positive correlation of α amylase with the
duration of disease and age.